Journal of Animal Behaviour and Biometeorology
Journal of Animal Behaviour and Biometeorology
Research Article Open Access

Effect of rearing, season of birth, and father on labyrinth behaviour of dairy heifers

Michal Uhrincat, Jan Broucek, Anton Hanus, Lucia Macuhova

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Objective of this study was to test the hypotheses that heifer's behaviour after 12 months (M) are impacted by rearing (feeding/housing) before weaning, seasons of birth, and father lineage. Fifty-one Holstein heifers (born in year seasons SB1, SB2, SB3, and SB4, originating from 4 fathers) were assigned to one of three rearing treatments: restricted suckling (RS), calf in pen with mother to 21st day, suck three times daily, then group pen (6 kg milk) to weaning; unrestricted suckling (US), calf in pen with foster cows (6 kg milk) to weaning; conventional rearing (CR), calf in the hutch to 56th day, then group pen to weaning (milk replacer 6 kg). After weaning at the 84th day, heifers were kept in groups with the same ration. The labyrinth behaviour was tested in the 12th and 19th M of the age. In the evaluation factors rearing and season of birth, groups US and SB3 solved the passage of the labyrinth the fastest (868.0 s, 857.4 s), the slowest were CR and SB1 (1148.2 s, 1257.5 s). The results show that the manner (housing/feeding) used to rear heifers and season of birth may impact their later labyrinth behaviour.


cattle, environment, learning, raising, welfare


Albright JL, Arave CW (1997) The Behaviour of Cattle. CAB International.

Anderson JF, Bates DW (1984) Clinical Cold Stress in Calves: Identification, Environmental Considerations, Treatment and Prevention. The Bovine Practitioners 19:22-25.

Angrecka S, Herbut P (2015) Conditions for cold stress development in dairy cattle kept in free stall barn during severe frosts.  Czech J Animal Science 60:81-87.  

Arave CW, Lamb RC, Arambel MJ, Purcell D, Walters JL (1992a) Behaviour and maze learning ability of dairy calves as influenced by housing, sex and sire. Applied Animal Behaviour Science 33:149-163.

Arave CW, Albright JL, Armstrong DV, Foster WW, Larson LL (1992b) Effects of isolation of calves on growth, behavior, and first lactation milk yield of Holstein cows. Journal of Dairy Science 75:3408-3415.

Barth K (2020) Effects of suckling on milk yield and milk composition of dairy cows in cow–calf contact systems. Journal of Dairy Research 87(S1):133-137.

Beaver A, Meagher RK, von Keyserlingk MAG, Weary DM (2019) A systematic review of the effects of early separation on dairy cow and calf health. Journal of Dairy Science 102:5784-5810.

Bell DJ, Robertson J, Macrae AI, Jennings A, Mason CS, Haskell MJ (2021) The Effect of the Climatic Housing Environment on the Growth of Dairy-Bred Calves in the First Month of Life on a Scottish Farm. Animals 11:2516.

Bilik K, Niwinska B, Lopusanszka-Rusek M, Fijal J (2013) Optimisation of calf rearing according to organic principles. Annals of Animal Science 13:341-355.

Boissy A, Bouissou MF (1995) Assessment of individual differences in behavioural reactions of heifers exposed to various fear-eliciting situations. Applied Animal Behaviour Science 46:17-31.

Bouissou MF, Boissy A, Le Neindre P, Veissier I (2001) The social behaviour of cattle. In: Keeling LJ, Gonyou HW (eds) Social Behaviour in Farm Animals. CAB International, Wallingford, UK, pp 113–145.

Braastad BO (1998) Effects of prenatal stress on behaviour of offspring of laboratory and farmed mammals.  Applied Animal Behaviour Science 61:159-180.

Broom DM, Fraser AF (2007) Domestic Animal Behaviour and Welfare. CAB International.

Broucek J, Kisac P, Uhrincat M (2003) The effect of sire line on learning and locomotor behaviour of heifers. Czech Journal of Animal Science 48:387-394.

Broucek J, Uhrincat M, Sandor A, Arave CW, Waiblinger S, Hanus A, Kisac P (2002) Einfluss eines niedrigen Magnetfeldes auf Kälber während der pränatalen Entwicklung. Tierärztliche Umschau 57:241-248.

Broucek J, Uhrincat M, Kisac P, Hanus A (2021) Effect of different rearing condition on early age in the maze, open-field, and aversive behaviours in the dairy cow during the first lactation. Journal of Animal Behaviour and Biometeorology 9:2117.

Budzynska M, Weary DM (2008) Weaning distress in dairy calves: effects of alternative weaning procedures. Applied Animal Behaviour Science 112:33–39.

Burman OHP, Parker RMA, Paul ES, Mendl M (2008) Sensitivity to reward loss as an indicator of animal emotion and welfare. Biology Letters 4:330–333.

Buttler L, Russ D, Wright C (2006) Cold Stress and Newborn Calves. Extension Extra. Paper 73, South Dakota State University.

Bünger U, Kaphengst P (1987) Zum Verhalten von Tränkkälbern in einer fremdartigen Umwelt. Monatshefte für Veterinärmedizin 42:378-383.

Carstens GE (1994) Cold thermoregulation in the newborn calf. Veterinary Clinics of North America: Food Animal Practice 10:69-106.

Collier RJ, Dahl GE, Van Baale MJ (2006) Major advances associated with environmental effects on dairy cattle. Journal of Dairy Science 89:1244-1253.

Coloma-García W, Mehaba N, Llonch P, Caja G, Such X, Salama AAK (2020) Prenatal heat stress effects on gestation and postnatal behavior in kid goats. PLoS One 15:e0220221.

Costa JHC, Daros RR, von Keyserlingk MAG Weary DM (2014) Complex social housing reduces food neophobia in dairy calves. Journal of Dairy Science 97:7804-7810.

Costa JHC, Meagher RK, von Keyserlingk MAG, Weary DM (2015) Early pair housing increases solid feed intake and weight gains in dairy calves. Journal of Dairy Science 98:6381-6386.

Costa JHC, von Keyserlingk MAG, Weary DM (2016) Effects of group housing of dairy calves on behavior, cognition, performance, and health. Journal of Dairy Science 99:2453-2467.

Dahl GE, Tao S, Laporta J (2020) Heat Stress Impacts Immune Status in Cows Across the Life Cycle. Frontiers in Veterinary Science 7:116. DOI: 10.3389/fvets.2020.00116

de Oliviera D, Barth K, Haskell MJ, Hillmann E, Jensen MB, Johnsen JF, Mejdell C, Weiblinger A, Ferneborg S (2020) Methodology for experimental and observational animal studies in cow-calf contact systems. Journal of Dairy Research 87:115-121.

de Passillé AM, Marnet PG, Lapierre H, Rushen J (2008) Effects of twice-daily nursing on milk ejection and milk yield during nursing and milking in dairy cows. Journal of Dairy Science 91:1416–1422.

de Paula Vieira A, de Passillé AM, Weary DM (2012) Effects of the early social environment on behavioral responses of dairy calves to novel events. Journal of Dairy Science 95:5149–5155.

European Commission (2007) Council Regulation (EC) No 834/2007 of 28 June 2007 on organic production and labelling of organic products and repealing Regulation (EEC) No 2092/91.

Flower FC, Weary DM (2001) Effects of early separation on the dairy cow and calf: 2. separation at 1 day and 2 weeks after birth. Applied Animal Behaviour Science 70:275-284.

Flower FC, Weary DM (2003) The effects of early separation on the dairy cow and calf. Animal Welfare 12:339-348.

Foris B, Zebunke M, Langbein J, Melzer N (2018) Evaluating the temporal and situational consistency of personality traits in adult dairy cattle. PLoS One 13:e0204619.

Fröberg S, Gratte E, Svennersten-Sjaunja K, Olsson I, Berg C, Orihuela A, Galina CS, Garcia B, Lidfors L (2008) Effect of suckling ('restricted suckling') on dairy cows' udder health and milk let-down and their calves' weight gain, feed intake and behaviour. Applied Animal Behaviour Science 113:1-14.

Gaillard C, Meagher RK, von Keyserlingk MAG, Weary DM (2014) Social housing improves dairy calves' performance in two cognitive tests. Plos One 9:e90205.

Gibbons JM, Lawrence AB, Haskell MJ (2010) Measuring sociability in dairy cows. Applied Animal Behaviour Science 122:84-91.

Gordon N (1997) Nutrition and cognitive function. Brain Development 19:165-170.

Graunke KL, Nürnberg G, Repsilber D, Puppe B, Langbein J (2013) Describing Temperament in an Ungulate: A Multidimensional Approach. PLoS One 8:e74579. DOI:10.1371/journal.pone.0074579.

Gräbner M, Kanitz E, Otten W (2009) Pränataler Stress bei Nutztieren: Eine Übersicht. Berliner und Münchener tierärztliche Wochenschrift 122:73-81.

Green AC, Johnston IN, Clark CEF (2018) Invited review: The evolution of cattle bioacoustics and application for advanced dairy systems. Animal 12:1250-1259.

Han CS, Dingemanse NJ (2015) Effect of diet on the structure of animal personality. Frontiers in Zoology 12:S5.

Hedlund L, Løvlie H (2015) Personality and production: Nervous cows produce less milk. Journal of Dairy Science 98:5819-5828.

Hempel S, Menz Ch, Pinto S, Galan E, Janke D, Estellés F, Müschner-Siemens T, Wang X, Heinicke J, Zhang G, Amon B, del Prado A, Amon T (2019) Heat stress risk in European dairy cattle husbandry under different climate change scenarios – uncertainties and potential impacts. Earth System Dynamics 10:859–884.

Herbut P, Angrecka S, Godyń D, Hoffmann G (2019) The physiological and productivity effects of heat stress in cattle - a review.  Annals of Animal Science 19:579-594.

Herbut P, Angrecka S, Walczak J (2018) Environmental parameters to assessing of heat stress in dairy cattle - a review. International Journal of Biometeorology 62:2089-2097. 

Herbut P, Hoffmann G, Angrecka S, Godyń D, Corrêa Vieira FM, Adamczyk K, Kupczyński R (2021) The effects of heat stress on the behaviour of dairy cows. Annals of Animal Science 21:385-402.

Hopster H, van der Werf JTN, Blokhuis HJ (1998) Side preference of dairy cows in the milking parlour and its effects on behaviour and heart rate during milking. Applied Animal Behaviour Science 55:213-229.

Horvath K, Fernandez M, Miller-Cushon EK (2017) The effect of feeding enrichment in the milk-feeding stage on the cognition of dairy calves in a T-maze. Applied Animal Behaviour Science 187:8-14.

Horvath K, Miller-Cushon EK (2018) Characterising social behavior, activity, and associations between cognition and behavior upon social grouping of weaned dairy calves. Journal of Dairy Science 101:7287-7296.

Jensen MB, Larsen LE (2014) Effects of level of social contact on dairy calf behavior and health. Journal of Dairy Science 97:5035-5044.

Jensen MB (1999) Effects of confinement on rebounds of locomotor behaviour of calves and heifers, and the spatial preferences of calves. Applied Animal Behaviour Science 62:43-56.

Johnsen JF, Passillé de AM, Mejdell CM, Boe KE, Grondahl AM, Beaver A, Rushen J, Weary DM (2015) The effect of nursing on the cow-calf bond. Applied Animal Behaviour Science 163:50-57.

Johnsen JF, Zipp KA, Kalber T, Passillé de AM, Knierim U, Barth K, Mejdell CM (2016) Is rearing calves with the dam a feasible option for dairy farms? Applied Animal Behaviour Science 181:1-11.

Johnsen JF, Holmoy IH, Nodtvedt A, Mejdell CM (2021) A survey of pre‑weaning calf management in Norwegian dairy herds. Acta Veterinaria Scandinavica 63:20.

Kent JP (2020) The cow–calf relationship: from maternal responsiveness to the maternal bond and the possibilities for fostering. Journal of Dairy Research 87(S1):101-107.

Kilgour R (1981) Use of the Hebb-Williams closed-field test to study the learning ability of Jersey cows. Animal Behaviour 29:850-860.

Kilgour R (1987) Learning and the Training of Farm Animals. Veterinary Clinics of North America: Food Animal Practice, Farm Animal Behavior 3:269-284.

Kälber T, Barth K (2014) Practical implications of suckling systems for dairy calves in organic production systems – a review. Landbauforschung-Applied Agricultural and Forestry Research 64:45-58.

Kovalcikova M, Kovalcik K (1987) Differences in the responses of heifers in the course of behavioural tests during ontogenesis. Zivocisna Vyroba 32:87-94.

Kovalcikova M, Kovalcik K, Broucek J (1988) The effects of the bull on the reactions and adaptability of the daughters in behavioural tests. Zivocisna Vyroba 33:201-209.

Köllmann K, Zhang Y, Wente N, Lücken A, Leimbach S, Krömker V (2021) Effects of Suckling on the Udder Health of Foster Cows. Ruminants 1:100-117.

Krachun C, Rushen J, de Passillé AM (2010) Play behaviour in dairy calves is reduced by weaning and by a low energy intake. Applied Animal Behaviour Science 122:71-76.

Krohn CC (2001) Effects of different suckling systems on milk production, udder health, reproduction, calf growth and some behavioural aspects in high producing dairy cows–a review. Applied Animal Behaviour Science 72:271-280.

Lapin M, Fasko P, Melo M, Stastny P, Tomlain J (2002) Climate areas. In: Atlas of Slovakia country. Ministry of Environment of the Slovak Republic and Slovak Environment Agency, Bratislava, pp 77- 94.

Laporta J, Fabris TF, Skibiel AL, Powell JL, Hayen MJ, Horvath K, Miller-Cushon EK, Dahl GE (2017) In utero exposure to heat stress during late gestation has prolonged effects on the activity patterns and growth of dairy calves. Journal of Dairy Science 100:2976-2984.

Latham NR, Mason GJ (2008) Maternal deprivation and the development of stereotypic behaviour. Applied Animal Behaviour Science 110:84-108.

Lauber MCY, Hemsworth PH, Barnett JL (2006) The effects of age and experience on behavioural development in dairy calves. Applied Animal Behaviour Science 99:41-52.

Lecorps B, Weary DM, von Keyserlingk MAG (2018) Pessimism and fearfulness in dairy calves. Scientific Reports 8:1421. DOI:10.1038/s41598-017-17214-3

Le Neindre P (1989) Influence of rearing conditions and breed on social behaviour and activity of cattle in novel environments. Applied Animal Behaviour Science 23:129-140.

Loberg J, Lidfors L (2001) Effect of stage of lactation and breed on dairy cows' acceptance of foster calves. Applied Animal Behaviour Science 74:97-108.

Loberg JM, Hernandez CE, Thierfelder T, Jensen MB, Berg Ch, Lidfors L (2008) Weaning and separation in two steps - A way to decrease stress in dairy calves suckled by foster cows. Applied Animal Behaviour Science 111:222-234.

Manteuffel G, Puppe B, Schön PC (2004) Vocalisation of farm animals as a measure of welfare. Applied Animal Behaviour Science 88:163-182.

Margerison JK, Phillips CJC, Preston TR, The effect of restricted suckling and nutrition on lactation, reproduction   and calf development. Proceedings British Society of Animal Science 37:62-65.

Meagher RK, Daros RR, Costa JHC, von Keyserlingk MAG, Hötzel MJ, Weary DM (2015) Effects of Degree and Timing of Social Housing on Reversal Learning and Response to Novel Objects in Dairy Calves. PLoS ONE 10:e0132828. doi: 10.1371/journal.pone.0132828

Meagher RK, von Keyserlingk MAG, Atkinson D, Weary DM (2016) Inconsistency in dairy calves' responses to tests of fearfulness. Applied Animal Behaviour Science 185:15-22.

Meagher RK, Beaver A, Weary DM, von Keyserlingk MAG (2019) A systematic review of the effects of prolonged cow–calf contact on behavior, welfare, and productivity. Journal of Dairy Science 102:5765-5783.

Meale SJ, Chaucheyras-Durand F, Berends H, Guan LL, Steele MA (2017) From pre- to postweaning: Transformation of the young calf's gastrointestinal tract. Journal of Dairy Science 100:5984-5995.

Meen GH, Schellekens MA, Slegers MHM, Leenders NLG, Van Erp-Van der Kooij E, Noldus LP (2015) Sound analysis in dairy cattle vocalisation as a potential welfare monitor. Computers and Electronics in Agriculture 118:111-115.

Melo M, Lapin M, Damborska I (2009) Methods for the design of climate change scenario in Slovakia for the 21st century. Bulletin of Geography. Physical Geography Series 1:77-90.

Miguel-Pacheco G, Vaughan A, de Passillé A, Rushen J (2015) Relationship between locomotor play of dairy calves and their weight gains and energy intakes around weaning. Animal 9:1038-1044.

Müller R, Schrader L (2005a) Individual Consistency of Dairy Cows' Activity in Their Home Pen. Journal of Dairy Science 88:171-175.

Müller R, Schrader L (2005b) Behavioural consistency during social separation and personality in dairy cows. Behaviour 142:1289-1306.

Müller R, von Keyserlingk MAG (2006) Consistency of flight speed and its correlation to productivity and to personality in Bos taurus beef cattle. Applied Animal Behaviour Science 99:193-204.

Neave HW, Costa JHC, Weary DM, von Keyserlingk MAG (2020) Long-term consistency of personality traits of cattle. Royal Society Open Science 7:191849.

Nonnecke BJ, Foote MR, Miller BL, Fowler M, Johnson TE, Horst RL (2009) Effects of chronic environmental cold on growth, health, and select metabolic and immunologic responses of preruminant calves. Journal of Dairy Science 92:6134-6143.

Novak P, Vokralova J, Tittl K, Mala G, Illek J (2010) Selected aspects of welfare and prevention of disease in ruminants. Veterinarstvi 60:25-27.

Purcell D Arave CW (1991) Isolation vs. group rearing in monozygous twin heifer calves. Applied Animal Behaviour Science 31:147-156.

Rashamol VP, Sejian V, Bagath M, Krishnan G, Archana PR, Bhatta R (2018) Physiological adaptability of livestock to heat stress: an updated review.  Journal of Animal Behaviour and Biometeorology 6:62-71.

Reenen van CG, van der Werf JTN, O'Connell NE, Heutinck LFM, Spoolder HAM, Jones RB, Koolhaas JM, Blokhuis HJ, O'Connell NE (2013) Behavioural and physiological responses of heifer calves to acute stressors: Long-term consistency and relationship with adult reactivity to milking. Applied Animal Behaviour Science 147:55-68.

Renaudeau D, Collin A, Yahav S, de Basilio V, Gourdine JL, Collier RJ (2012) Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal 6:707-728.

Roland L, Drillich M, Klein-Jöbstl D, Iwersen M (2016) Influence of climatic conditions on the development, performance, and health of calves. Journal of Dairy Science 99:2438-2452.

Roman L, Morales Pineyrua JT, Banchero G, La Manna A (2021) Access to shade during the dry period improves the performance of multiparous Holstein cows. Animal Production Science 61:1706-1714.

Romeyer A, Bouissou MF (1992) Assessment of fear reactions in domestic sheep, and influence of breed and rearing conditions. Applied Animal Behaviour Science 34:93-119.

Roth BA, Barth K, Gygax L, Hillmann E (2009) Influence of artificial vs. mother-bonded rearing on sucking behaviour, health and weight gain in calves. Applied Animal Behaviour Science 119:143-150.

Rushen J, de Passillé AM (1998) Behaviour, welfare and productivity of dairy cattle. Canadian Journal of Animal Science 78(Suppl):3-21.

Rushen J, de Passillé AMB, von Keyserlingk MAG, Weary DM (2008) The welfare of cattle. Springer, Dordrecht, Netherlands

Schrader L (2002) Consistency of individual behavioural characteristics of dairy cows in their home pen. Applied Animal Behaviour Science 77:255-266.

Schuster AC, Carl T, Foerster K (2017) Repeatability and consistency of individual behaviour in juvenile and adult Eurasian harvest mice. The Science of Nature 104:10. DOI:10.1007/s00114-017-1430-3

Shetty PS (1999) Adaptation to low energy intakes: the responses and limits to low intakes in infants, children and adults. European Journal of Clinical Nutrition 53(Suppl 1):S14-S33.

Siebert K, Langbein J, Schön PC, Tuchscherer A, Puppe B (2011) Degree of social isolation affects behavioural and vocal response patterns in dwarf goats (Capra hircus). Applied Animal Behaviour Science 131:53–62.

Slavik P, Illek J, Brix M, Musilova L, Rajmon R, Klabanova P, Jilek F (2009) Health Status of Beef Cows and their Calves in the Czech Republic. Acta Veterinaria Brno 78:47-56.

Steele M (2019) Performance and Behavioural Effects of Separating Dairy Cows and Their Calves at Birth. Veterinary Evidence 4:24.

Stehulova I, Lidfors L, Spinka M (2008) Response of dairy cows and calves to early separation: effect of calf age and visual and auditory contact after separation. Applied Animal Behaviour Science 110:144-165.

Stehulova I, Spinka M, Sarova R, Machova L, Knez R, Firla P (2013) Maternal behaviour in beef cows is individually consistent and sensitive to cow body condition, calf sex and weight. Applied Animal Behaviour Science 144:89-97.

Tao S, Bubolz JW, do Amaral BC, Thompson IM, Hayen MJ, Johnson SE, Dahl GE (2011) Effect of heat stress during the dry period on mammary gland development. Journal of Dairy Science 94:5976-5986.

Tao S, Monteiro APA, Thompson IM, Hayen MJ, Dahl GE (2012) Effect of late-gestation maternal heat stress on growth and immune function of dairy calves. Journal of Dairy Science 95:7128-7136.

Tao S, Dahl GE (2013) Heat stress effects during late gestation on dry cows and their calves. Journal of Dairy Science 96:4079-4093.

Uhrincat M, Broucek J, Hanus A, Kisac P (2021) Effect of Raising Dairy Heifers on Their Performance and Reproduction after 12 Months. Agriculture 11:973.

Valnickova B, Stehulova I, Sarova R, Spinka M (2015) The effect of age at separation from the dam and presence of social companions on play behaviour and weight gain in dairy calves. Journal of Dairy Science 98:5545-5556.

Veissier I, Le Neindre P (1992) Reactivity of Aubrac heifers exposed to a novel environment alone or in groups of four. Applied Animal Behaviour Science 33:11-15.

Veissier I (1993) Observational learning in cattle. Applied Animal Behaviour Science 35:235-243.

Ventura G, Lorenzi V, Mazza F, Clemente GA, Iacomino C, Bertocchi L, Fusi F (2021) Best Farming Practices for the Welfare of Dairy Cows, Heifers and Calves. Animals 11:2645.

von Keyserlingk MAG, Weary DM (2007) Maternal behaviour in cattle: A review. Hormones and Behavior 52:106-113.

von Keyserlingk MAG, Rushen J, de Passillé AM, Weary DM (2009) The welfare of dairy cattle-key concepts and the role of science. Journal of Dairy Science 92:4101-4111.

Wagenaar JPTM, Langhout J (2007) Practical implications of increasing 'natural living' through suckling systems in organic dairy calf rearing. NJAS - Wageningen Journal of Life Sciences 54:375-386.

Wagner K, Seitner D, Barth K, Palme R, Futschik A, Waiblinger S (2012) Integration into the dairy cow herd: Long-term effects of mother contact during the first twelve weeks of life. Applied Animal Behaviour Science 141:117-129.

Wagner K, Barth K, Hillmann E, Palme R, Futschik A, Waiblinger S (2013) Mother rearing of dairy calves: reactions to isolation and to confrontation with an unfamiliar conspecific in a new environment. Applied Animal Behaviour Science 147:43-54.

Wagner K, Seitner D, Barth K, Palme R, Futschik A, Waiblinger S (2015) Effects of mother versus artificial rearing during the first 12 weeks of life on challenge responses of dairy cows. Applied Animal Behaviour Science 164:1-11.

Wechsler B, Lea SEG (2007) Adaptation by learning: Its significance for farm animal husbandry. Applied Animal Behaviour Science 108:197-214.

Winder CB, Bauman CA, Duffield TF, Barkema HW, Keefe GP, Dubuc J, Uehlinger F, Kelton DF (2018) Canadian National Dairy Study: Heifer calf management. Journal of Dairy Science 101:1-15.

Zhang C, Juniper DT, Meagher RK (2021) Effects of physical enrichment items and social housing on calves' growth, behaviour and response to novelty. Applied Animal Behaviour Science 237:105295.

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